| Course ID: | BBL-SE>TCTC |
| Course title: | TECHNIQUES OF CELL AND TISSUE CULTURES |
| Semester: | 2 / Spring |
| ECTS: | 3 |
| Lectures/Classes: | 15 / 30 hours |
| Field of study: | Biology |
| Study cycle: | 2nd cycle |
| Type of course: | compulsory |
| Prerequisites: | Histology, Genetics, Biochemistry, Physiology |
| Contact person: | dr hab. inż. Wojciech Kruszyński, prof. nadzw. wojciech.kruszynski@upwr.edu.pl |
| Short description: | The course contains the issues involving the principles of tissue and cell cultures of selected mammalian tissues. |
| Full description: | Material used in cytogenetics and kind of tissue cultures, plant cell and tissue cultures, animal cell and tissue cultures, usefulness of cell and tissue culture in biology, medicine and veterinary medicine, preparation and banding chromosome slides (conventional stain and banding stain, FISH), karyograms and their analysis, cytogenetic diagnostic of animals and human, techniques of banding of germ cells. |
| Bibliography: | 1. Freshney R. I. 2005. Culture of Animal Cells: A Manual of Basic Techniqu. Wiley. Nowy Jork 2. Mather J.P.1998. Introduction to Cell and Tissue Culture. Theory and Technique, Springer |
| Learning outcomes: | Knowledge: W1 – has the knowledge allowing to interpret the natural phenomena and processes in research work and practical activities, knows the research methodology obligatory in natural sciences, formulates research hypotheses and solves basic scientific problems, knows how to create an interesting multimedia presentation W2 – knows methods of chemical and instrument al analysis, with special attention paid to physico-chemical basis, has the knowledge of research methods and techniques used in laboratories, knows the rules of biological material handling, has the knowledge essential for laboratory equipment operating, demonstrates advantages and disadvantages of particular analytical techniques W3 – characterizes techniques, methods and application of cell and tissue cultures of plant and animal organisms, describes the rules of cytogenetic preparations making, their staining and proper analysis. Skills: U1 – effectively and safely uses research apparatus and measurement devices used in biology, is able to select suitable measurement devices and measure needed values of physiological and morphological features U2 – uses the methods applied in laboratory analytics: optical, spectroscopic, electroanalytical, separation ones, is able to assess factors influencing the result of laboratory analysis avoiding pre- and post-analytical mistakes, interprets the results of laboratory analysis based on standards and reference values, collects and interprets the data form analysis and formulates conclusions based on them U3 – operates various types of microscopes, is able to make the preparations using light and electron microscopy, performs histological staining of the preparations, is able to use an application of SEM-EDS microscopy in determination of elemental composition of the examined substance Social competences: K1 – demonstrates creativity and is able to determine priorities in task realization taking into account team work K2– obeys the rules of occupational health and safety in biological laboratories, demonstrating responsibility for an assessment of dangers resulting from research techniques used, demonstrates the care of creation of safe work conditions. |
| Assessment methods and assessment criteria: | Completion of the course: total credit course (classes and lectures) based on the average of the marks obtained from two written tests and assessments of oral statements during exercise. Written tests (two tests, lasting 20-30 minutes) include issues discussed during classes and lectures. The presence at exercises is obligatory; student can have one unexcused absence. In the absence excused student is required to pass the relevant part of the material. On completion it is necessary to obtain 60% of the points |